IRWIN FRIDOVICH. The Department of Biochemistry, Duke University Medical Center,. Durham, North Carolina Received April 21, Superoxide. Beauchamp, C. and Fridovich, I. () Superoxide Dismutase Improved Assays and an Assay Applicable to Acrylamide Gels. Analytical Biochemistry, Author: Beauchamp C, Journal: Analytical biochemistry[/11] Fridovich I. Find all citations in Analytical Biochemistry [01 Nov , 44(1)]. /.

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The advantage of this technique is that the assay is not modified by the introduction of new reagents. No difference in the quantitative reliability was found in any riboflavin concentration.

Nagwa Abdallah Ismail, Sawsan H. Fine-tuning reagent concentrations to effectively visualize bands continue to be a major research obstacle in assay development. The reduction of nitroblue tetrazolium by superoxide radicals generated from photo-reactive riboflavin has been in use for more than four decades to detect superoxide dismutase SOD on nondenaturing polyacrylamide gels.

Treatment with single doses frivovich these agents time dependently increased testicular MDA, decreased SOD level and induced abnormal testicular histopathological changes.

Testicular levels of malondialdehyde MDAsuper oxide dismutase SOD and histopathological changes were also analyzed. Assay sensitivity was assessed by comparing lowest possible visible enzyme load of the experimental stains with the 0. Due to lack of significant synergistic effects on all evaluated parameters with the co-administration of these agents, the concurrent use of these agents in the management of HIV and co- infections fridovch not have any deleterious effect on male reproductive function.


Advances in Enzyme ResearchVol. Scientific Research An Academic Publisher. Animals were sacrificed at the end of treatment, testes were collected and weighed. Okasha, Anil Dhawan, Azza M.

The concurrent use of these drugs may place more adverse burden on testicular function because reports have associated these drugs individually with decreased testicular function. The correlation R2 values of trial averages were compared against the average correlation of the standard 0. This technique has already been employed to analyze SOD protein expression levels in extracts of Escherichia coli Bertrand et al. Yan Li, Nathaniel P. Analytical Biochemistry, 44, beauchanp Sperm count, sperm motility, and morphology were evaluated.

Low micromolar to low millimolar concentrations of yellow riboflavin can be mixed with the blue of reduced nitroblue tetrazolium to controllably produce blue, purple, yellow-brown, or yellow gel backgrounds. Quantitative reliability of these alternative stains was assessed by plotting determined band intensity values against known enzyme loads.


SOD research in medicine and biochemistry has warranted the development of multiple assay variants to overcome specific experimental constraints or to combine the SOD assay with other enzyme assays. Glutathione peroxidase, superoxide dismutase and catalase activities in children with chronic hepatitis. Eighty 80 adult male rats which were divided beaucchamp five 5 groups A-E were used in this study.

Superoxide dismutase: improved assays and an assay applicable to acrylamide gels.

Budi Handono, Firman F. The minimum reliable sensitivity of the assay was found to be 10 ng for each concentration of riboflavin. Mose, Tri Hanggono Achmad. Herein we describe a straightforward technique to reliably adjust the background color of polyacrylamide gels without compromising assay efficacy.

No significant synergistic effects were observed in all evaluated parameters when these agents were co-administered.